ABSTRACT: This study presents results of integrated larger foraminiferal biostratigraphy, lithofacies and stable carbon isotope (13 C org) analysis carried out in the Sylhet Limestone of Dillai Parbat Limestone Mine succession, Karbi Anglong District, Assam. Larger foraminiferal assemblages assign a middle Eocene age to the studied succession of Sylhet Limestone. The Shallow Benthic Zone (SBZ) 13 and SBZ 16-18 and a barren interval in between is recognized in the studied part of the Sylhet Limestone. The SBZ 13 Zone (early Lutetian) is represented by fossiliferous limestone at the base of the section and is inferred to have been deposited in a subtidal environment. The overlying barren interval lies in the arenaceous interval and was likely deposited in a subtidal channel sand shoaling bars. The upper part of the section ismarked by SBZ16-18 (late Lutetian to Bartonian) and was deposited in subtidal channel sand inner neritic zone environments, followed by intertidal marl to subtidal limestone. The organic carbon stable isotopic data reveals a sharp negative excursion at about 34-35m of the succession, which may be correlated with Middle Eocene Climatic Optimum (MECO) and/or vegetation types. This and earlier studies from India and elsewhere indicate that during the middle Eocene, the Neo-Tethys Sea was open and connected through the East and West coasts of India, Northeast India, western Himalaya, and most of the Middle East and southeast Europe. This vast area produced enormous amount of petroleum.