ABSTRACT: The establishment of a high-frequency sequence stratigraphic framework (short-term cycles) is the basis of seismic sedimentology research, which provides a new way to establish a high-resolution isochronous stratigraphic framework using 3D-seismic data. The linear interpolation between reference seismic reflections is used to establish a high-resolution isochronous stratigraphic framework (stratal slices). By carefully calibrating time-depth relationships, a corresponding relationship between short-term cycle (high-frequency cycle) interfaces and stratal slices is created. Five reference seismic reflections correspond to maximum flood surfaces. The results show that 311 isochronous stratal slices are formed in the 90 degree phase of the 3D seismic data set. Reference seismic reflection does not change with frequency. The event axis of reference for isochronous seismic reflection often corresponds to the most obvious isochronous interfaces. This method can establish a high-resolution isochronous stratigraphic framework in areas lacking drilling data in a 3D seismic data set. When geological dating data is available, the stratal slices can be further calibrated to absolute geological time, and a paleogeological map can be constructed from the seismic data set. This case study also illustrates the theoretical and practical significance of this method.