A biostratigraphic integration study based on the planktonic foraminifera and calcareous nannofossils from the Mango-2 well, North El Fayrouz Offshore Field, Mediterranean Sea, Egypt, provides a well-defined zonal scheme for the Oligocene–middle Miocene interval. The lithologic succession was subdivided into three formations, from oldest to youngest; Sidi Salem, Qantra and Tineh. Six planktic foraminiferal zones and four calcareous nannofossils zones, in addition to two unconformities are identified. The planktic foraminiferal zones include Ciperoella angulisuturalis, Paragloborotalia opima opima (Oligocene), Globigerinoides primordius, Praeorbulina curva (early Miocene), Praeorbulina glomerosa and Fohsella peripheroronda (middle Miocene). The calcareous nannofossil zones include Furcatolithus distentus, (Oligocene), Discoaster druggii, Helicosphaera ampliaperta (early Miocene) and Sphenolithus heteromorphus (middle Miocene). The absence of the Paragloborotalia kugleri, Paragloborotalia pseudokugleri and Furcatolithus ciperoensis zones suggests an unconformity across the Oligocene–Miocene boundary. Another unconformity was identified across the Aquitanian–Burdigalian boundary based on the absence of the Globoquadrina dehiscens, Globigerinita dissimilis, Globigerinatella insueta and Sphenolithus belemnos zones. These unconformities are most likely related to regional tectonics.